Protein Function - Enzymes

1. General Comments
   1. Enzymes
   2. Specificity
   3. Interaction
      1. active site
         1. common features
         2. lock & key
         3. induced fit
      2. temperature effect
      3. pH effect
         1. cholinesterase
         2. trypsin
         3. papain
2. Free Energy - G
   1. free energy (G)
   1. Two thermodynamic properties of rxns.
      1. free energy change (DG)
         1. exergonic
         2. endergonic
      2. activation E
      1. Reaction Coordinate Graph
         1. E hill
         2. transition state
      2. Remember:
   2. DG and its relation to the Equilibrium Constant
      1. DG = DG° + RTln[B]/^[A]
         1. Standard free-energy change (DG°)
         2. biochemical standard free E change = (DG°')
      1. ex. DHAP l GAP
      2. Notes:
3. Enzyme Classes
   1. Oxidoreductases
   2. Transferases
   3. Hydrolases
   4. Lyases
      1. synthase
   5. Isomerases
   6. Ligases
      1. sythetases