Protein Function - Enzymes

   I. General Comments
      A. Enzymes
      B. Specificity
      C. Interaction
         1. active site
            a. common features
            b. lock & key
            c. induced fit
         2. temperature effect
         3. pH effect
            a. cholinesterase
            b. trypsin
            c. papain

  II. Free Energy - G
             1)      free energy (G)
      A. Two thermodynamic properties of rxns.
            a. free energy change (DG)
               1) exergonic
               2) endergonic
            b. activation E
         1. Reaction Coordinate Graph
               1) E hill
               2) transition state
         2. remember
      B. delta G and its relation to the Equilibrium Constant
               1) delta G = delta Gų + RTln[B]/[A]
                  a) Standard free-energy change (delta Gų)
                  b) biochemical standard free E change = (delta Gų')
            a. ex. DHAP l GAP
            b. Notes

 III. Common features of Active sites

  IV. Enzyme Classes
      1. Oxidoreductases
      2. Transferases
      3. Hydrolases
      4. Lyases
         a. synthase
      5. Isomerases
      6. Ligases
         a. sythetases