1. Hub Reaction
   1. acetyl-CoA
   2. pyruvate dehydrogenase (PDH) complex
      1. size
      2. cofactors
      3. enzymes
         1. pyruvate dehydrogenase - E1
         2. dihydrolipoyl tranacetylase - E2
         3. dihydrolipoyl dehydrogenase - E3
2. Reactions
   1. Decarboxylation
   2. Oxidation
   3. formation of acetyl CoA
3. Beriberi disease