Enzyme Kinetics

1. Enzyme Kinetics
   1. Saturation kinetics
      1. First order kinetics
      2. Zero order kinetics
   2. Enzyme Substrate Complex
      1. E + S <=> ES  E + P
         1. rates (k)
      2. Evidence for ES complex
   3. Compare & Contrast
      1. Sames
      2. Differences
      3. Effects of Huge S
      4. Initial velocity = V₀
2. Mickalis-Menton Equation
   1. Variables
      1. V_max
      2. V_o
      3. K_m
   1. Finding Km
   2. Assumptions
   3. Using the Affinity Constant
      1. Example
   4. General rate constant = kcat
      1. k_cat = turn over number
3. Lineweaver-Burk plot
   1. Derivation
   2. Plot = 1/V_o verses 1/[S]
      1. = double reciprocal plot