Enzyme Kinetics

   I. Enzyme Kinetics
      A. Saturation kinetics
            a. First order kinetics
            b. Zero order kinetics
      B. Enzyme Substrate Complex
         1. E + S <=> ES -> E + P
            a. rates (k)
         2. Evidence for ES complex
      C. Compare & Contrast
         1. Sames
         2. Differences
         3. Effects of Huge S
         4. Initial velocity = V0

  II. Mickalis-Menton Equation
         1. Variables
            a. Vmax
            b. Vo
            c. Km
      A. Finding Km
      B. Assumptions
      C. Using the Affinity Constant 
         1. Example
      D. General rate constant = kcat
         1. kcat = turn over number

 III. Lineweaver-Burk plot
      A. Derivation
      B. Plot = 1/vo verses 1/[S]
         1. = double reciprocal plot