Antibodies and Antigens                      Chapter 5

   I. Introduction
      A. Antibodies = Ab
      B. Antigens = Ag
      C. Location
      D. Blood Ab vocabulary
         1. plasma
         2. serum
         3. serology
         4. antiserum
         5. titer

  II. Molecular Structure of Abs
      A. General features of Ab structure
         1. Immunoglobulins (Ig)
         2. Ig domain
      B. Side to side
         1. heavy chains
         2. light chains
            a. lambda, kappa
      C. Top to Bottom
         1. Function
            a. Fab
            b. Hinge
            c. Fc
         2. Sequence
            a. Variable region
               1) hypervariable regions
                  a) CDRs
            b. Constant region
               1) membrane bound
      D. Classes = isotypes
         1. IgM
         2. IgG
            a. colostrum
         3. IgA
            a. MALT
            b. J chain
         4. IgD
         5. IgE

 III. Antibody Binding of Antigens
      A. Features of Biologic Antigens
         1. immunogen
         2. antigen
            a. hapten
         3. epitope
      B. Structural and Chemical Basis of Ag Binding
         1. affinity,  Kd

  IV. Monoclonal Abs
      A. Credit
      B. Defined
      C. Procedure - hybridoma
      D. Uses
      E. Polyclonal Abs