Cell Communication Chapter 16

1. General Principles
   1. Binding Specificity - ability of receptor to distinguish closely related substances
   2. Cellular response
2. Intracellular Receptors
3. Cell Surface receptors
   1. Overview
   2. Classes
      1. Ion-channel-coupled receptors
      2. G-protein-coupled receptors
      3. Enzyme-coupled receptors
4. Ion-channel-coupled Receptors
5. G Protein-coupled Receptors
   1. Components
   2. Receptor Structure
   3. G Protein part of GTPase Superfamily
      1. Molecular functioning
         1. Guanine nucleotide Exchange Factor (GEF)
      2. Two subfamilies
         1. Trimeric
         2. Monomeric
   4. Trimeric G proteins
      1. Structure
      2. Activation
      3. Deactivation
   5. Toxins
      1. Vibrio cholera
      2. Bordetella pertussis
   6. G protein Effector Proteins
      1. adenylyl cyclase
         1. cAMP phosphodiesterase
         1. Protein Kinase A
      2. phospholipase C
         1. PIP2
            1. DAG
            2. IP3
         2. IP3
            1. calmodulin
         3. DAG
            1. protein kinase C
      3. ion channels
   7. Down regulationEnzyme Coupled Receptors
   8. Overview
6. Receptor Tyrosine Kinases (RTKs)
   1. Structure
      1. dimers
      2. Off
   2. Ras / Raf Pathway
      1. GRB2
         1. SH2 domain
         2. SH3 domain
      2. Sos
      3. Ras
      4. Raf
      5. MAP kinase kinase kinase
      6. MAP kinase kinase
      7. MAP kinase
      8. transcription factors
      9. Turning off Ras
         1. GAP
   3. Phosphoinositide 3-kinase
      1. Protein kinase B (PKB) = Akt
      2. Bad
7. Receptors with no enzymatic activity
   1. JAK / STAT pathway
      1. receptor
      2. JAK
      3. STAT
      4. Off
         1. SOCS
   1. Protein cleavage
      1. NF-kB & I-kB