Cytoskeleton              Chapter 17a

   I. Cytoskeleton

  II. Intermediate filaments
      A. Background
      B. Location
         1. cytoplasm
         2. nucleus = nuclear lamina
      C. Structure
         1. monomer - protein domains
         2. dimer
         3. tetramer
         4. octamers 
         5. filament formation
      D. Function
      E. Classes
         1. cytoplasmic
            a. keratin
            b. vimentin & vemintin related filaments
            c. neurofilaments
         2. nuclear = nuclear lamina
            a. lamin
            b. progeria
      F. Additional proteins 
         1. plectin

 III. Microtubules
      A. Background
      B. Structure
         1. tubulin
            a. heterodimer
               1) alpha-tubulin
               2) beta-tubulin
         2.polarized
      C. Tubule formation
         1. centrosome
         2. contents
            a. centrioles
            b. centrosome matrix
               1) gamma-tubulin
                  a) nucleation site
         3. basel bodies
      D. Tubule growth
         1. half-life of tubule
         2. dynamic instability
         3. observational
            1) catastrophe
            2) rescue
         4. molecularly
            1) GTP cap
         5. overview
         6. drugs
            a. lab
               1) colchicine & nocodazole
               2) nivblastine & vincristine
               3) taxol
      E. Microtubule-associated proteins
      F. Cilia & Flagella
         1. background
         2. structure
            a. basal body
            b. transition zone 
            c. axonome 
               1) 9 + 2
         3. movement
            a. dynein
         4. Kartagener's syndrome

  IV. Actin Filaments
      A. Overview
      B. Protein structure
         1. actin
            a. G-actin
            b. F-actin
      C. Filament structure
         1. polarity
      D. Cellular functions
      E. Growth
      F. in vitro Polymerization
         1. nucleation phase
         2. elongation phase
         3. steady-state phase
         4. treadmilling
      G. Actin binding proteins
         1. thymosin & profilin 
         2. formins