Transcription

1. Nucleic Acids
   1. Differences
   2. Gene
      1. coding region
   3. Central Dogma of Molecular Biology
   4. Template
   5. RNA structure
      1. hairpins
      2. stem-loop
2. Structure Overview
   1. @ DNA level
      1. +1
      2. upstream = 5'
      3. downstream = 3'
   2. @ RNA level
      1. transcript
      2. 5' & 3'
   3. Error rate
3. Transcription Overview
   1. initiation
      1. transcription bubble
   2. strand elongation
      1. pyrophosphate
      2. stability
   3. termination
4. Types of RNA
   1. Regulating RNA molecules
      1. micro RNAs = miRNA
         1. RISC
      2. snRNA
      3. lncRNA
   2. siRNA
      1. RNAi
         1. dicer
            1. RITSTranscription and Gene Structure
   3. Amplification
   4. Prokaryotic transcription
      1. RNA polymerase
         1. sigma factor
      2. promoter
      3. terminator
      4. operon
         1. polycinstronic
   5. Eukaryotic transcription
      1. Transcription-control regions
         1. promoter
         2. control elements
         3. enhancers
      2. RNA polymerases
      3. general transcription factors
         1. TFIID
         2. TFIIB
         3. TFIIH
      4. Enhancers
5. RNA Processing
   1. Prokaryotic
      1. polycistronic
   2. Eukaryotic
      1. monocistronic
      1. RNA cap
         1. process
         2. why
      2. polyadenylation
         1. sequences
         2. cleavage/polyadenylation complex
            1. poly(A) polymerase (PAP)
         3. poly(A) tail
      3. RNA splicing
         1. introns & exons
            1. recognition seqs
         2. spliceosome
            1. snRNAs
            2. snRNPs
         3. process
            1. lariat structure
         4. alternative splicing
      4. export
         1. export proteins
6. RNA degradation
   1. Half life
   2. Pathways of mRNA degradation
      1. deadenylation
      2. deadenylation-independent decapping pathway
      3. endonucleolytic pathway
   3. Additional control
      1. miRNAs
      2. siRNAs
      3. half life control