The Control of Microbial Growth Chapter 7 I. Terminology A. Nosocomial B. Sterilization 1) commercial sterilization C. Disinfection a. disinfectant b. antiseptic D. Sanitization E. Degerming F. -cide G. -stat or -stasis H. Sepsis I. Asepsis II. Rate of Death A. Factors III. Physical Methods of Control A. Heat 1. moist heat a. boiling b. autoclave c. pasteurization 1) Milk standards a) Classic, HTST, UHT 2. dry heat B. Filtration C. Low Temp D. Dessication E. Osmotic Pressure F. Radiation a. ionizing radiation b. nonionizing radiation - U.V. 1) thymine dimers IV. Chemical Methods of Control A. Evaluation 1. use-dilution test 2. disk-diffusion method - Kirby-Bauer a. zone if inhibition B. Types of Disinfectants 1. phenol a. phenolics b. bisphenols 2. biguanides 3. halogens a. iodine 1) tincture 2) iodophor b. chlorine 4. alcohols 5. heavy metals a. oligodynamic action 6. surfactants a. soaps and detergents b. acid-anionic sanitizers c. Quats 7. chemical food preservatives a. organic acids 8. antibiotics 9. aldehydes 10. gaseous sterilants 11. plasmas 12. oxidizing agents a. peroxygens b. ozone