Microbial Metabolism                              Chapter 5

_Student Required Vocabulary_
            Phototrophs
            Chemotrophs
            Autotrophs
            Heterotrophs
         Now put the terms together
            Chemoheterotroph
            Chemoautotroph
            Photoheterotroph
            Photoautotroph

_Lecture Outline_

   I. Reactions
      A. Metabolism
         1. anabolism
         2. catabolism
         3. exchange reaction

  II. Enzymes
      A. Collision theory
         1. activation E
      B. Catalyst
      C. Enzyme structure
         1. substrates
         2. active site
         3. products
      D. Naming
      E. Cofactors
         1. apoenzyme
         2. holoenzyme
         3. cofactors
            a. coenzyme
               1) NAD+, NADP+, FAD, coenzyme A
      F. Activity
      G. Inhibitors
         1. competitive
         2. noncompetitive
            a. allosteric
         3. feedback inhibition

 III. Energy Use Comparison

  IV. Exchange Reaction Energy
      A. Energy-carrier molecules
         1. ATP
         2. electron carriers
      B. Pathways

   V. Carbohydrate Catabolism
      A. Cellular respiration
      B. Fermentation

  VI. Glycolysis
      A. Notes
      B. Glucose activation
            a. fructose 1,6 bisphosphate
      C. Energy harvest 
            a. glyceraldehyde 3-phosphate
         1. pyruvate

 VII. Cellular Respiration
      A.Decarboxylation - Hub reaction 
            a. acetyl CoA
      B. Krebs cycle
         1. Notes
         2. Process
         3. Krebs Totals
      C. Electron transport chain
         1. notes
         2. process
         3. chemiosmosis
            a. proton motive force
         4. ATP synthase
         5. subtotals
         6. etc Totals

VIII. Final totals
      A. Substrate-level phosphorylation
      B. Oxidative phosphorylation

  IX. Anaerobic fermentation
      A. Overview
      B. Glycolysis
      C. pyruvate
         1. regenerate NAD+ from NADH
      D. Fermentation products
         1. alcohol
         2. acids
            a. butyric acid
            b. propionic acid
            c. lactic acid

   X. Photosynthesis
      A. 6 CO2 + 6 H2O + solar E  ®  C6H12O6 + 6 O2
         1. C fixation