Student Responsible Material

• Units of Measure
• Metric system

1. The Instruments
   1. Simple
   2. Compound
2. Illumination
   1. Light Microscopy
      1. Resolution
      2. Refractive index
         1. Oil
      3. Brightfield
      4. Darkfield
      5. Phase contrast
      6. DIC
   2. Fluorescence
   3. Confocal
   4. Electron Microscopy
      1. TEM
      2. SEM
   5. Scanning probe microscopes
3. Specimen Preparation
   1. Staining Preparation
      1. Staining
      2. Fixing
      3. Smear
   2. Stain composition
      1. Basic
      2. Acidic
   3. Simple stain
      1. mordant
   4. Differential stain
4. Gram stain
   1. Procedure
   2. gram positive
   3. gram negative
5. Acid-Fast stain
   1. Procedure
   2. Orgs
6. Special structure staining
   1. Negative
   2. Endospore
   3. Flagella