Microscopy                         Chapter 3

_Student Responsible Material_
Units of Measure
Metric system

_Lecture Outline_

  I. The Instruments
      A. Simple
      B. Compound

 II. Illumination
      A. Light Microscopy
         1. Resolution
         2. Refractive index
            a. Oil
         3. Brightfield
         4. Darkfield 
         5. Phase contrast
         6. DIC
      B. Fluorescence
      C. Confocal
      D. Electron Microscopy
         1. TEM
         2. SEM
      E. Scanning probe microscopes

 III. Specimen Preparation
      A. Staining Preparation
         1. Staining
         2. Fixing
         3. Smear
      B. Stain composition
         1. Basic
         2. Acidic
      C. Simple stain
         1. mordant
      D. Differential stain

  IV. Gram stain
      A. Procedure
      B. gram positive
      C. gram negative

   V. Acid-Fast stain
      A. Procedure
      B. Orgs

  VI. Special structure staining
      A. Negative
      B. Endospore
      C. Flagella