Chapter 5 - Enzyme Technology

1. The Nature of Enzymes
   1. Properties
      1. catalyst
      2. nontoxic
      3. biodegradable
      4. specificity
   2. Source
   3. Definition
   4. Cofactors
   5. Industrial uses
2. The Application of Enzymes
   1. Historical location
      1. downsides
      2. amount needed
   2. Crude enzymes
      1. problem
   3. Extracellular enzymes
   4. Intracellular enzymes
   5. Enzymes in detergents story
   6. Prices
      1. causes
      2. result
   7. Markets
   8. Amounts
      1. bulk
         1. source
      2. diagnostic
3. Selection and Development of Producer Strains for Enzyme Production
   1. Screening & selection
   2. Improvements
      1. past - Random mutations
      2. now - Protein engineering
         1. define
         1. steps
      3. objectives Table 5-6
      4. when done
         1. preproduction
         2. post production
4. The Technology of Enzyme Production
   1. Source
      1. reasons
   2. Goals
      1. constituent mutants
   3. Uses
      1. substrates
      2. production
   4. Forms
   5. Safe product goals
   6. Safety testing Table 5.7
5. Immobilized enzymes
   1. Soluble enzymes
   2. Immobilization
      1. process Table 5.8
         1. physical
         2. chemical
      2. advantages Table 5.9
   3. Present industrial processes/products
   4. Diagnostic use
   5. Enzyme electrodes