Chapter 12 - Stem Cell Biotechnology 12.1 The nature of stem cells A. Cellular differentiation 1. definition 2. process B. Adult stem cells C. Types of stem cells 1. totipotent 2. pluripotent a. blastocyst b. embryonic stem cells 3. multipotent D. History 1. first isolation 2. adult stem cells a. successes b. predictions E. Other sources 12.2 Stem cell cultivation A. Requirements 1. suitable media 2. substratum B. Growth C. Challenge D. Production requirements 1. GMP E. Transcription factors 12.3 Human-animal embryos A. Why B. Solution C. Process D. Use 12.4 Commercial potential for stem cell therapies A. Success B. Money